Table 4.
Performance of three direct LDL-C assays with detection of cholesterol on purified Lp(a) and Lp(a) spiked-in plasma
| Input |
% Pure Lp(a)-C detected | % Spiked-in Lp(a)-C detected | |||
|---|---|---|---|---|---|
| Plasma sample | Purified Lp(a) | Plasma + purified Lp(a) | |||
| TC | 155.6 (3.2) | 54.9 (1.3) | 200.5 (0.6) | 100 | 100 |
| Roche direct LDL-C | 97.7 (2.2) | 44.4 (0.6) | 144.0 (1.1) | 87 (3.1) | 84 (2.3) |
| Sekisui direct LDL-C | 113.1 (6.4) | 57.3 (1.9) | 166.8 (3.3) | 104 (5.4) | 98 (0.2) |
| Wako direct LDL-C | 105.1 (5.2) | 49.7 (2.3) | 158.9 (2.9) | 90 (6.2) | 98 (0.1) |
Purified Lp(a) with a cholesterol content of 54.9 mg/dl was used for these experiments. % pure Lp(a)-C detected = Lp(a)-C measured by the respective direct LDL-C assay divided by Lp(a)-C measured by the TC assay. % spiked-in Lp(a)-C = cholesterol measured in plasma spiked with pure Lp(a) − cholesterol measured in plasma without added Lp(a) divided by cholesterol measured in purified Lp(a), using each respective assay. Data described are from three separate experiments and expressed as mean (SD). Units are in milligrams per deciliter unless otherwise specified.