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. 2021 Feb 23;62:100051. doi: 10.1016/j.jlr.2021.100051

Fig. 4.

Fig. 4

4β-HC induces lipid-droplet formation and triglyceride accumulation. A: 4β-HC increases the lipid droplet size and number. Huh7 cells were treated with 5 μM 4β-HC with indicated drugs for 72 h followed staining with lipid droplet dye, BODIPY 493/503, and visualization by confocal microscopy and (B) quantified using ImageJ (N = 3). C: 4β-HC increases triglycerides (TAG) levels. Huh7 cells were treat as (C) followed by measurement of triglycerides, total cholesterol, and protein levels using commercial kits (N = 3). D: 4β-HC increases lipid droplet in the mouse liver. Mice were fed normal chew with either vehicle 50 mg/kg/day 4β-HC or 10 mg/kg/day GW3965 for 5 days. Liver samples were fixed and stained with BODIPY 493/503 and DAPI to observe lipid droplet and nuclei ultrastructure (N = 3). E: 4β-HC increases triglycerides (TAG) levels in the mouse liver treated as above, followed by measurement of triglycerides, total cholesterol, and protein levels using commercial kits (N = 3). For convenience, agonists are marked in green and antagonists are marked in red. Bars are the mean + SD. Statistical significance calculated by one-way ANOVA.∗P < 0.05, ∗∗P < 0.01, ∗∗∗∗P < 0.0001. ent-4HC, stereo enantiomer-4HC, FASNi, TVB-3166; LXRi, GSK-2033; 4β-HC, 4β-hydroxycholesterol.