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. 2021 Apr 8;23(4):429–438. doi: 10.1016/j.neo.2021.03.005

Fig. 3.

Fig. 3

TNBC lung colonization is not dependent on Akt activation. (A) MDA-MB-231 GFP cells were treated with 10 μM MK-2206 for 22 h. Fresh medium with 10 μM MK-2206 was replaced 2 h before cells were collected and intravenously injected into NSG mice. Expression of pAkt (Ser473) was analyzed by western blot. (B) MDA-MB-231 lung metastasis was visualized with fluorescence imaging 46 d after cell injection. (C) MDA-MB-231 GFP cells were treated with 10 μM AZD5363 for 22 h. Fresh medium with 10 μM AZD5363 was replaced 2 h before cells were collected and intravenously injected into NSG mice. Expression of pAkt (Ser473) was analyzed by western blot. (D) MDA-MB-231 lung metastasis was visualized with fluorescence im aging 45 d after cell injection. (E) Analysis of Akt1, cleaved PARP, and Bim expression in MDA-MB-231 cells treated with siAkt1 or MDA-MB-231 GFP cells treated with 10 μM MK-2206 or AZD5363 from (A) and (C), respectively, by western blot. (F) Analysis of Akt2 expression in MDA-MB-231 GFP cells treated with 10 μM MK-2206 or AZD5363 from (A) and (C), respectively, by western blot.