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. 2021 Mar 15;7(4):811–825. doi: 10.1021/acsinfecdis.0c00676

Table 11. Primers and Oligonucleotides Used in This Study.

name sequence description
p282 AACATATGTTAAATATTTATTTCTC for genotyping of recombinant pfmfr3 locus
p283 AGGGTTATTGTCTCATGAGCGG for genotyping of recombinant pfmfr3 locus
p1277 TGACAGATATCCTGTGGAAGATATCG for genotyping of recombinant pfmfr3 locus
p1281 GTGAGGCAAATGTATTTATTATACC for genotyping of recombinant pfmfr3 locus
F1_mfr3_avrII ATCGCCTAGGATGAAAAAAGTAAAGG for amplification of full length pfmfr3 cDNA
R1_mfr3_mfeI ATCGCAATTGTTACATTTGCTGTAG for amplification of full length pfmfr3 cDNA
F2_mfr3_mfeI ATCGCAATTGGATATATATCTTTAGTG for amplification of full length pfmfr3 cDNA
R2_mfr3_nheI ATCGGCTAGCCTTTGAAGAAGGAAGGG for amplification of full length pfmfr3 cDNA
BSD_F ATCAACAGCATCCCCATCTC for amplification of the BSD resistance cassette
BSD_R ATGCAGATCGAGAAGCACCT for amplification of the BSD resistance cassette
mfr3_GFP_F TCACCTTCACCCTCTCCACT for amplification of the pfmfr3-GFP junction on the overexpression/tagging episome
mfr3_GFP_R CCAAAGGCAATAGCTCAAGG for amplification of the pfmfr3-GFP junction on the overexpression/tagging episome
rrs_qpcr_F GAGTACCCCAATCACCTACA for qPCR (ΔΔCt), reference
rrs_qpcr_R AAGAGATGCATGTTGGTCATTT for qPCR (ΔΔCt), reference
mfr3_qpcr_F CCAAAGGCAATAGCTCAAGG for qPCR (ΔΔCt), target
mfr3_qpcr_R TTGAAGAAGGAAGGGAAATCA for qPCR (ΔΔCt), target