p282 |
AACATATGTTAAATATTTATTTCTC |
for genotyping of recombinant pfmfr3 locus |
p283 |
AGGGTTATTGTCTCATGAGCGG |
for genotyping of recombinant pfmfr3 locus |
p1277 |
TGACAGATATCCTGTGGAAGATATCG |
for genotyping of recombinant pfmfr3 locus |
p1281 |
GTGAGGCAAATGTATTTATTATACC |
for genotyping of recombinant pfmfr3 locus |
F1_mfr3_avrII |
ATCGCCTAGGATGAAAAAAGTAAAGG |
for amplification of full
length pfmfr3 cDNA |
R1_mfr3_mfeI |
ATCGCAATTGTTACATTTGCTGTAG |
for amplification of full
length pfmfr3 cDNA |
F2_mfr3_mfeI |
ATCGCAATTGGATATATATCTTTAGTG |
for amplification of full
length pfmfr3 cDNA |
R2_mfr3_nheI |
ATCGGCTAGCCTTTGAAGAAGGAAGGG |
for amplification of full
length pfmfr3 cDNA |
BSD_F |
ATCAACAGCATCCCCATCTC |
for amplification of the
BSD resistance cassette |
BSD_R |
ATGCAGATCGAGAAGCACCT |
for amplification of the
BSD resistance cassette |
mfr3_GFP_F |
TCACCTTCACCCTCTCCACT |
for amplification of the pfmfr3-GFP junction on the overexpression/tagging episome |
mfr3_GFP_R |
CCAAAGGCAATAGCTCAAGG |
for amplification of the pfmfr3-GFP junction on the overexpression/tagging episome |
rrs_qpcr_F |
GAGTACCCCAATCACCTACA |
for qPCR (ΔΔCt),
reference |
rrs_qpcr_R |
AAGAGATGCATGTTGGTCATTT |
for qPCR (ΔΔCt),
reference |
mfr3_qpcr_F |
CCAAAGGCAATAGCTCAAGG |
for qPCR (ΔΔCt),
target |
mfr3_qpcr_R |
TTGAAGAAGGAAGGGAAATCA |
for qPCR (ΔΔCt),
target |