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. 2021 Apr 2;45(6):88. doi: 10.3892/or.2021.8039

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — CREB is upregulated in LUAD. (A) UALCAN database was used to analyze TCGA data of CREB expression between LUAD (n=515) and normal lung (n=59) tissues. (B) CREB expression in lung fibroblast, LUSC and LUAD cell lines, as measured by IB and IHC. (C and D) CREB expression in LUAD and adjacent normal tissues was measured by ELISA, as presented in a (C) line plot and (D) scatter plot. (E) CREB expression in 10 pairs of LUAD tissues with the most significant increase of CREB according to the ELISA data of C and D, as measured by IB and qPCR. (F) CREB expression in 3 pairs of LUAD tissues, as measured by IHC (scale bar, 800 µm). The data are presented as the mean ± SD from three biological replicates (including IB). **P<0.01 indicates statistical significance. Data from A were analyzed using an independent-sample Student's t-test, D and E were analyzed using paired Student's t-test. CREB, cAMP response element-binding protein; LUAD, lung adenocarcinoma; TCGA, The Cancer Genome Atlas; LUSC, lung squamous cell carcinoma; IB, immunoblotting; IHC, immunohistochemistry; ELISA, enzyme-linked immunosorbent assay.