Fig. 6.

PD-L1-lnc binds to c-Myc and activates c-Myc signal downstream. a The heatmap of dysregulated genes in A549 cells transfected with PD-L1-lnc-expressing vector (PD-L1-lnc), PD-L1-lnc shRNA vector (PD-L1-lnc shRNA), or control vectors (CTL Vector). b Transcriptional factor analysis among the upregulated or downregulated genes in A549 cells following PD-L1-lnc overexpression or PD-L1-lnc knockdown. The bars reflect the percent of dysregulated genes regulated by the transcriptional factor. c Expression level of genes within c-Myc signaling downstream in A549 cells with PD-L1-lnc overexpression (Right) or knockdown (Left). d Immunoprecipitation of PD-L1-lnc in A549 cells using biotinylated antisense probes against PD-L1-lnc to detect the PD-L1-lnc-associated c-Myc. e Immunoprecipitation of c-Myc in A549 cells using anti-c-Myc antibody and ProteinA/G beads to detect the c-Myc-associated PD-L1-lnc. *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.0001