Figure 2.

HIOs Maintain Their Regional Identity after SARS-CoV-2 Infection

(A) qRT-PCR for phenotypic intestinal markers at 1 and 3 dpi colonic HIOs (2^−ΔΔCT, technical duplicates normalized to GAPDH, n = 3 independent directed differentiation experiments, error bars represent the SD, statistical significance where indicated determined by unpaired Student's t test, ^∗∗ = p < 0.01).

(B) Local regression (locally estimated scatterplot smoothing [LOESS]) plots profiling regional-specific (colonic/proximal) markers in mock (left) and infected (right) HIOs.

(C) Representative confocal immunofluorescent micrographs of infected colonic HIOs showing maintained expression of Villin, at 1 and 4 dpi.

(D) Representative confocal immunofluorescent micrographs of infected proximal HIOs showing maintained expression of Villin, at 1 and 4 dpi. (All scale bars represent 50 μm, images representative of n = 3 replicate directed differentiations).

See also Figures S1 and S2.