Figure 3.

Transmission Electron Microscopy of SARS-CoV-2-Infected Colonic and Proximal HIOS

Representative electron micrographs of (A) proximal HIO enterocytes infected with SARS-CoV-2 showing large numbers of coronavirus particles adjacent to the cell surface (inset, white arrowheads), (B) proximal HIO cytoplasm with budding virions (inset, white arrowheads), (C) infected colonic HIOs with intracellular replication complexes (white arrowheads) and viral particles within a single membrane vesicle (inset), and (D) cytoplasmic regions of viral replication with characteristic horseshoe structures and budding virions (inset). Scale bars, 500 nm (A, C, D large images), 200 nm (B, large image), or 125 nm (all insets); images representative of n = 3 replicate directed differentiation experiments. See also Figure S2.