Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 12;9:88. doi: 10.1186/s40168-021-01043-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 5 — Identification of Pir^VP genes in isolated colonies from ILI strain. a PCR characterization of ILI’s individual colonies (1-23) and positive control (lane 24; V. parahaemolyticus BA94C2). PCR targets the Pir^VP genes coded in the plasmid from BA94C2. Notice that all strains were positive for the plasmid except two (lanes 20 and 21). EPM: DL2000 DNA marker. b Shrimp cumulative mortality after being challenged with transconjugant colonies of the ILI strain and V. parahaemolyticus BA94C2 strains. Negative control was TSB 2% NaCl. Bars indicate standard deviations (n = 5 replicates, see methods for the number of shrimps per assay). c ILI’s individual colonies recovered from hepatopancreas and stomachs of surviving shrimp challenged with transconjugant colonies of the ILI strain. Lane 1: V. parahaemolyticus BA94C2 containing the Pir^VP genes; lanes 2–8: ILI’s individual colonies; lane 9: V. parahaemolyticus BA94C2 containing the Pir^VP genes; lane 10: ILI colony not containing the Pir^VP genes; and lane 11: PCR negative control; EPM: DL2000 DNA marker