Fig. 5.

Transcriptome analyses. a Heatmap and hierarchical tree comparing the global transcriptome differences in edited (∆45–55/Cas9, ∆46–54/Cas9, ∆46–54/Cas12a and INDEL50/Cas9, n = 2) and negative control (Cas9 only, mock and unedited, n = 2) myotubes differentiated from the DMD–MDSCs with wild-type (WT, n = 4) myotubes differentiated from normal MDSCs. Red and green color intensities represent gene upregulation and downregulation, respectively. Gene ontology (GO) enrichment results of two clustered gene sets are shown on the right side of the heatmap; −log10 p values were used to produce the bar plots. b Gene Ontology analysis of transcripts in edited myotubes