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. 2021 Apr 13;21:204. doi: 10.1186/s12935-021-01898-9

Fig. 6.

Fig. 6

Flow cytometry analysis of CRC-CSC markers expression in HT-29 and Caco-2 spheroids compared to their parental cells. Representative flow cytometry plots confirmed both HT-29 and Caco-2 spheroids show higher expression of CD166, CD44 and CD133 CRC-CSC markers as compared to their parental cells. Upregulation of CSC markers in spheroids as compared to their differentiated counterparts was predominant in Caco-2 spheroids than HT-29 spheroids. Dot plots show the expression of each marker in one representative experiment