Table1.
Primers used for quantitative RT-PCR
| Genes groups | Gene name | Primer Sequence (5´ → 3´) |
|---|---|---|
| Housekeeping gene | GAPDH |
F-CATGAGAAGTATGACAACAGCCT R-AGTCCTTCCACGATACCAAAGT |
| Stemness genes | C-MYC |
F-ACACATCAGCACAACTACG R-CGCCTCTTGACATTCTCC |
| KLF4 |
F-CCTCGCCTTACACATGAAGAG R-CATCGGGAAGACAGTGTGAAA |
|
| SOX2 |
F-AATGGGAGGGGTGCAAAAGAGG R-GTGAGTGTGGATGGGATTGGTG |
|
| NANOG |
F-AGCTACAAACAGGTGAAGAC R-GGTGGTAGGAAGAGTAAAGG |
|
| OCT4-A |
F-GTGGAGAGCAACTCCGATG R-TGCAGAGCTTTGATGTCCTG |
|
| EMT genes | Vimentin |
F-TCTACGAGGAGGAGATGCGG R-GGTCAAGACGTGCCAGAGAC |
| SNAIL1 |
F-CCAGAGTTTACCTTCCAGCA R-GATGAGCATTGGCAGCGA |
|
| TWIST1 |
F-TTCTCGGTCTGGAGGATGGA R-CCACGCCCTGTTTCTTTGAAT |
|
| N-cadherin |
F-GCCCAAGACAAAGAGACCC R-CTGCTGACTCCTTCACTGAC |
|
| E-cadherin |
F-CAGGAGTCATCAGTGTGGT R-GGAGGATTATCGTTGGTGTCAG |
|
| ZEB1 |
F-CTTCTCACACTCTGGGTCTTATTC R-CGTTCTTCCGCTTCTCTCTTAC |
|
| ABC Transporte | ABCG2 |
F-TTCCACGATATGGATTTACGG R-GTTTCCTGTTGCATTGAGTCC |
| ABCB1 |
F-GTTCAGGTGGCTCTGGATAAG R-AGCGATGACGTCAGCATTAC |
|
| ABCC1 |
F-CGCCTTCGCTGAGTTCCT R-TGCGGTGCTGTTGTGGTG |