Table 2.

The expression percentage of CD44, CD166, and CD133 common CRC-CSCs markers in spheroids compared to their parental cells

Markers	Cell types
	HT-29		Caco-2
	Parental	Spheroid	Parental	Spheroid
CD44%	1.80 ± 0.77	19.3 ± 6.60*	1.59 ± 0.28	41.05 ± 3.53***
CD133%	1.35 ± 0.19	11.75 ± 4.69*	1.25 ± 0.45	28.25 ± 17.45*
CD166%	1.66 ± 0.37	22.40 ± 5.92*	1.78 ± 0.18	37.15 ± 4.02***

Data are shown as mean ± SD of four independent experiments. A significant upregulation of CSC markers in spheroids compared to parental cells are presented as * = P < 0.05, *** = P < 0.001, ns = not significant