Table I.
Lubricin quantification in human joint disease. ELISA – enzyme-linked immunosorbent assay, IHC – immunohistochemistry, WB – Western blot, MS – mass spectrometry, SF – synovial fluid, OA – osteoarthritis, NA – not available, mo – months, ACL – anterior cruciate ligament, eOA – early osteoarthritis, lOA – late osteoarthritis.
| Injury | Assay |
Time of sampling | Control | Outcome | ||||
|---|---|---|---|---|---|---|---|---|
| ELISA | IHC | WB | MS | |||||
| 39 | Tibial plateau fracture | ✓ | 11 +/−9 days postinjury | Contralateral knee SF (n=3); healthy human knee SF (n=3) | [Lubricin] increased post-injury | |||
| 40 | Knee OA (varus malalignments) | ✓ | End stage OA | Lateral “spared compartment” cartilage in varus knee joints (n=3) | Lubricin staining increased in OA | |||
| 41 | Total knee arthroplasty | ✓ | ✓ | ✓ | End stage OA | Histologically healthy knee cartilage; cartilage from radiographically normal cadaver knees (n=3) | [Lubricin] increased in OA; lubricin staining increased in more severely affected areas of joint | |
| 42 | Total knee arthroplasty | ✓ | End stage OAOA | Observational OA progression cohort knee SF (n=173) | Lubricin increased with decreasing joint space in age/sex-adjusted model | |||
| 43 | Total knee arthroplasty | ✓ | End stage | Calf and adult bovine cartilage (n=NA) | Lubricin staining increased in OA | |||
| 59 | Tibial plateau fracture | ✓ | 12–66 mo postinjury | Contralateral knee SF (n=10) | [Lubricin] unchanged | |||
| 60 | Total arthroplasty, knee and hip | ✓ | End stage OA | Histologically normal areas of same menisci and labra (n=NA) | Lubricin staining unchanged | |||
| 61 | Tibial osteotomy; total knee arthroplasty; meniscectomy with OA | ✓ | End stage OA; chronic OA | Healthy human knee SF (n=NA) | [Lubricin] unchanged; correlated with anteroposterior laxity, full flexion angle and range of knee motion | |||
| 37 | ACL injury | ✓ | 32–364 days postinjury | Contralateral knee SF (n=30) | [Lubricin] decreased post-injury | |||
| 62 | eOA; lOA | ✓ | eOA (arthroscopy) and lOA (arthroplasty) | Human cadaver knee SF (n=16) | [Lubricin] decreased in lOA | |||
| 38 | Meniscal injury | ✓ | 2 days post-injury | Human cadaver menisci (n=9) | Lubricin staining decreased post-injury | |||
| 14 | Meniscectomy with OA | ✓ | ✓ | NA | Human menisci and SF obtained 3 months post-ACL injury (n=9) | Lubricin expression decreased in OA menisci and OA SF lavage samples | ||