Figure 5.

WD40 improves the activity of hydrophobic and nonplanar antibiotics in P. aeruginosa (strain PA14). (A) Bar graph showing MIC fold changes for antibiotics in combination with WD40. The names of drugs are colored blue for those with reported antipseudomonal activity. To identify which physicochemical properties are best predictors for potentiation, dot plots were generated of MIC fold change versus (B) molecular weight, (C) ClogP, (D) globularity, (E) plane-of-best-fit (PBF), and (F) number of rotating bonds. (B–F) Drugs with reported antipseudomonal activity are indicated by blue dots. Only small molecule antibiotics were included in correlation analysis. Therefore, colistin and vancomycin are excluded from the shown graphs. Pearson’s correlation coefficients (r) and p-values were calculated using linear regression analysis. The results were reproduced in two independent experiments. For each experiment, n = 1 per antibiotic.