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. 2021 Apr 6;22(3):248–256. doi: 10.1080/15384047.2020.1863120

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Figure 1. — TDRG1 is prominently up-regulated in BC and knockdown of TDRG1 impedes malignant behaviors of BC progression in vitro

A．QRT-PCR analysis was utilized to evaluate the TDRG1 expression level in BC cell lines and normal epithelial cell line (MCF-10A). B. The efficiency of TDRG1 silencing was ensured by qRT-PCR analysis. C-D. CCK8 and CFSE assays were conducted to test cell proliferation ability after knockdown of TDRG1. E. Flow cytometry was utilized to assess apoptosis after knockdown of TDRG1. F-G. Transwell assays were implemented to assess the number of migrated and invaded cells after knockdown of TDRG1. H. Tube formation assay was implemented to detect angiogenesis after knockdown of TDRG1 in HUVECs. **P < .01.