Figure 2.

TDRG1 exerts oncogenic properties in BC via negatively regulating miR-214-5p

A-B. Subcellular fractionation and FISH assay were conducted to determine the location of TDRG1. C. QRT-PCR analysis was implemented to investigate miRNA expression when TDRG1 was silenced. D. QRT-PCR analysis was performed to detect the expression profile of miR-214-5p in cell lines. E. The binding sites of miR-214-5p with TDRG1 were predicted. F. Wild and mutant TDRG1 sequences were sub-cloned into luciferase reporter. Luciferase activities were normalized to renilla luciferase. G. RNA pull down was performed to explore the mechanical relationship between miR-214-5p and TDRG1. H. The inhibiting efficiency of miR-214-5p inhibitor was evaluated by qRT-PCR. I-N. Rescue experiments were performed to evaluate the impact of miR-214-5p inhibitor on sh-TDRG1induced BC biological activities. **P < .01.