Figure 2.

Feeding habit affects glucose homeostasis in zebrafish. Postprandial blood glucose (a) and insulin levels (b) in zebrafish. Adult zebrafish (2-month-old) were fed CD, OD and HD with antibiotic mixture (Polymyxin B 2.5 g/kg diet and Neomycin 3.3 g/kg diet) for 1 week. Postprandial blood glucose (c) and insulin (d) in zebrafish after antibiotic treatment. The intestinal microbiota of CD, OD and HD-treated zebrafish were transferred to GF zebrafish. Free glucose (e) and the relative expression of insulin (f) of GF zebrafish inoculated with intestinal bacteria of CD, OD and HD treated zebrafish. Data were expressed as the mean ± SEM (n = 3 biological replicates). *p < .05; **p < .01; ***p < .001; ****p < .0001