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. 2021 Apr 6;7(5):748–756. doi: 10.1021/acscentsci.1c00197

Figure 3.

Figure 3

Production of m1Ψ mRNAs by in vitro transcription. Left: Components of in vitro transcription reaction. Right: Incorporation of m1Ψ-triphosphate into RNA is guided by m1Ψ’s ability to form a canonical base pair with adenine of the DNA template in the T7 RNA polymerase active site.