One mm2 of cutaneous tissue was excised, weighed, and homogenized at days 3, 5, and 7 following application of daily topical IMQ cream. Samples were stained with fluorescence-labeled mAb for (A) Ly6-G+ (neutrophils), (B) F4/80+ (macrophages), and (C) CD4+ (T-cells) cells. Bars represent the mean values; error bars indicate standard deviations. Asterisks denote P-value significance (P < 0.05) calculated using student’s t-test analysis. The experiments were performed twice with similar results obtained.