Homogenates of excised cutaneous lesions from control mAb IgG2a and mAb 1A8 mice 3, 5, and 7 days post-IMQ topical application were prepared and the supernatants were analyzed for (A) TNF-α, (B) IFN-γ, (C) IL-1β, (D) IL-4, (E) IL-6, and (F) IL-10 levels. Bars represent the mean values; error bars indicate standard deviations. Symbols (* lower; # higher) denote P-value significance (P < 0.05) calculated using student’s t-test analysis. Cytokine quantification was performed thrice with similar results obtained.