FIGURE 2.
High molecular weight kininogen (HK) contributes to thrombin generation and inflammation in sickle cell disease. Wild type (WT) and HK−/− mice were lethally irradiated and transplanted with bone marrow from AA (n = 15–22) and SS mice (n = 25–28). Four months later plasma was collected for analysis of (A) thrombin-antithrombin (TAT), (B) interleukin 6 (IL-6), and (C) soluble vascular cell adhesion molecular (sVCAM). Data are presented as mean ± standard error of the mean and were analyzed by two-way analysis of variance and Tukey’s multiple comparison post-hoc analysis. *P < .05 and ***P < .001 versus AA/WT. Asterisks above lines indicate significance between SS/HK−/− and SS/WT