FIGURE 6.

Long-term high molecular weight kininogen (HK) deficiency attenuates tubular injury in sickle mice. Urine was collected for 24 hours from AA/WT, AA/KO, SS/WT, and SS/KO mice 240 days after bone marrow transplant and analyzed for (A) osmolality and (B) kidney injury marker-1 (KIM-1) levels normalized to creatinine. Data are presented as mean ± standard error of the mean (SEM). C, Quantification of interstitial fibrosis index score and (D) representative images of Picro Sirius-red stained sections. Original magnification ×20; scale bar = 100 µm. E, Quantification of F4/80⁺-stained area and (F) representative images of F4/80⁺-stained glomerular sections. Original magnification ×40; scale bar = 50 µm. G, Quantification of brush border loss index score and (H) representative images from hematoxylin and eosin-stained sections. Original magnification ×120; scale bar = 50 µm. (I) Quantification of Prussian blue staining representing iron deposition and (J) representative images of Prussian blue stained sections. Original magnification ×40; scale bar = 50 µm. Data are presented as mean ± SEM, and analyzed by two-way analysis of variance. *P < .05, **P < .01, ***P < .001 and ****P < .0001 versus AA/WT. Asterisks above lines indicate difference from SS/WT