Immunofluorescent labelling of VPL and VPM. Mild-TBI altered the immunoreactive expression of 5-HT (b), DBH (e) and NK1R (h) in the VPL and VPM (B: −3.2) area of cortex according to the Paxinos atlas compare to the corresponding areas of control animals (a, d, g). Scale bar = 50 μm. A quantitative evaluation of fiber density for 5-HT (c), DβH (f) and NK1R expressing cells (i) showed significant elevation following m-TBI. “**”, indicates p < 0.005 and “***”, indicates P < 0.0005.