Table 3. Determination of pathogenicity of the isolated S. agalactiae (GBS) in the healthy Tilapia and V. Koi fishes by aquarium-based experimentally induced infection.

Experimental groups	Hosts	No. of fishes used per trial	Bacterial isolate	Routes and doses of inoculated bacteria and PBS			Number and % mortality of fishes (Day 1-day 6 of p.i.)			Number and % of sick/recovered fishes (Day 7-day 14)
				Oral (0.1 ml/dose of 7 × 107 CFU/ml)	IP (0.1 ml/dose of 1 × 107 CFU/ml)	Mock control group IP injection of PBS (0.2 ml/ dose)	Oral	IP	Mock control group (PBS)	Oral	IP	Mock control group (PBS)
1st trial	for Tilapia	15	S.agalactiae (GBS) (field isolate)	5	5	5	4 (80%)	5 (100%)	0	1 (20%)	0 (0%)	0
2nd trial		15		5	5	5	5 (100%)	5 (100%)	0	0 (0%)	0 (0%)	0
3rd trial		15		5	5	5	4 (80%)	5 (100%)	0	1 (20%)	0 (0%)	0
1st trial	for V. Koi	15		5	5	5	4 (80%)	5 (100%)	0	1 (20%)	0 (0%)	0
2nd trial		15		5	5	5	4 (80%)	5 (100%)	0	1 (20%)	0 (0%)	0
3rd trial		15		5	5	5	5 (100%)	5 (100%)	0	0 (0%)	0 (0%)	0

IP indicates the IP route of inoculation; p.i. Indicates post-infection; CFU indicates colony-forming unit; The routes and doses of bacterial (GBS) inoculum used in the experimental infection of this study were followed as per the routes and doses used by Iregui et al. [22] and Pereira et al. [24].