Table 1. List of primers and probes used for identification of Matrix (M) gene of avian influenza and its subtypes.

Target gene	Item	Name	Sequence	Reference
M	Forward	IVA D161M	5’ AGATGAGYCTTCTAACCGAGGTCG 3’	[46]
	Reverse	IVA D162M1	3’ GTCTCTGAACTYCTACAAAAACGT 5’
		IVA D162M2	3’ GTCTCTGAACTYCTACACAAACGT 5’
		IVA D162M3	3’ GTCTCTGAACTYCTACAGAAACGT 5’
		IVA D162M4	3’ GTCTCTGAACTYCTACATAAACGT 5’
	Probe	IVA-MA	5’-FAM-TCAGGCCCCCTCAAAGCCGA-TAMRA-3’
H5	Forward	IVA D148H5	5’ AAACAGAGAGGAAATAAGTGGAGTAAAATT 3’
	Forward	IVA D204f	5’ ATGGCTCCTCGGRAACCC 3’
	Reverse	IVA D149H5	3’ CGTTAGTACCATCGACCAGATAGAAA 5’
	Reverse	IVA D205r	3’ GCCTTACCAGAATGTATCACCTYTT 5’
	Probe	IVA H5a	5’-FAM-TCAACAGTGGCGAGTTCCCTAGCA-TAMRA-3’
	Probe	IVA D215P	5’-FAM-ATGTGTGACGAATTCMT-MGBNFQ-3’
H9	Forward	H9	5’ ATGGGGTTTGCTGCC 3’	[47]
	Reverse	H9v2 Rev3	3’ ACGTCYACGTTGTAAACATATA 5’
	Probe	H9v2 ProbeV4	5’-FAM-TTCTGGGCYATGTCCAATGG-BHQ-1-3’
N1	Forward	IAV-N1-3-F	5’ AGRCCTTGYTTCTGGGTTGA 3’
	Reverse	IAV-N1-3-R	3’ ACCAGAACCGGTCTGCCA 5’
	Probe	IAV-N1-3-FAM	5’FAM-ATYTGGACYAGTGGGAGCAGCAT-BHQ1 3’
N2	Forward	IAV-N2-1367F	AGT CTG GTG GAC YTC AAA YAG
	Reverse	IAV-N2-1488R	AAT TGC GAA AGC TTA TAT AGV CAT
N6	Probe	IAV-N2-1444.1FAM-MGB	FAM-CCA TCA GGC CAT GAG CCT-MGB
N6	Forward	IAV-N6-10F	5’ AGG GTG AAR ATG AAT CCA AAY CA 3’
	Forward	IAV-N6-14F	3’ TGA ARA TGA ATC CAA ATC AGA AGA TAA 5’
	Reverse	IAV-N6-97R	3’ CATCATTCRGACGAYTATCCTAAC 5’
	Probe	IAV-N6-43FAM	5’-FAM-TGC ATH TCA GCH ACA GGA ATG ACA CTA TC-BHQ1-3’
N8	Forward	IAV-N8-1296F	5’ TCCATGYTTTTGGGTTGARATGAT 3’
	Reverse	IAV-N8-1423R	3’ ACCAGYACCGTRCTACCTCG 5’
	Probe	IAV-N8-1354FAM	5’-FAM-TCHAGYAGCTCCATTGTRATGTGTGGAGT-BHQ1-3’