Skip to main content
. 2021 Apr 13;16(4):e0249748. doi: 10.1371/journal.pone.0249748

Fig 7. Wnt5a/b protein expression is up-regulated in the transected rat nerve.

Fig 7

A-O, Immunohistochemical staining of Wnt5a/b (green) and nuclear staining with DAPI (blue) on transversal sections through the proximal stump (left column), injury site (middle column) and distal stump (right column) of the rat peripheral nerve at 3 to 90 days after nerve transection. Wnt5a/b expression is low in the proximal stump at all time points (A, D, G, J, M). The transection- coaptation site shows high Wnt5a/b staining at 3 days (B) gradually diminishing in staining intensity over time from 5 (E), 14 (H), to 28d p.i. (K) and returns to baseline at 90d p.i. (N). In the distal stump Wnt5a/b expression is increased at 3 (C), 5 (F), 14 (I) and 28d p.i. (L) and has returned to baseline at 90d p.i. (O). Quantification of Wnt5a/b signal in the proximal stump, injury site and distal nerve stump is shown in the graphs in panels P to T. (* p<0.05, ** p<0.01, *** p<0.001, error bars represent SEM). Scale bar is 25μm.