Figure 5 |. Vaccination with polymersome-formulated RBD improves antigen-specific T cell responses in mice.
Cells isolated from the injection-site draining lymph nodes of PS-vaccinated mice 1 week post-boost were restimulated ex vivo for 6 h with RBD peptide pools or full RBD protein and analyzed by flow cytometry or multiplexed ELISA, respectively. a-b, percentages of cytokine-positive CD8+ T cells (a) and CD4+ T cells (b) in response to RBD peptide pools, subtracting unstimulated controls. c, Pro-inflammatory cytokine levels released into the supernatant measured after 3 d restimulation with whole RBD protein. Data plotted as mean ± SD and represent 1 of 2 experiments with n = 5 mice each. Symbols represent individual mice. Comparisons to unadjuvanted RBDfree were made using one-way ANOVA with Dunn’s post-test.