Protein lysates of right lung lobe tissue collected from mice at necropsy were assayed by Western blotting for STAT proteins and β-actin. A) Representative Western blots of lung tissue from WT or Stcit6 KO mice exposed to vehicle, HDM, MWCNTs, or HDM and MWCNTs. B)Densitometry of STAT1 signal normalized against the β-actin signal. #P < 0.05 between genotypes, one-way ANOVA. N=5 animals per group. C)Densitometry of p-STAT3 normalized against total STAT3. *P < 0.05 compared to vehicle, Mann-Whitney test. D)Densitometry of total STAT3 normalized against β-actin. N=4 to 6 animals per group.