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. 2020 Dec 21;2(2):162–185. doi: 10.1158/2643-3230.BCD-20-0029

Figure 2.

Figure 2.

MYC directly suppresses TFEB expression to control TFEB target genes. A, TFEB promoter-luciferase reporter activity, normalized to Renilla luciferase, in HL60 cells following CRISPR-directed MYC knockout (KO) or the induction of TFEB–ERT2 activity with 4OHT. B–F, MYC-HA–expressing OCI-AML3 cells that were engineered to inducibly express TFEBS211A-FLAG were incubated with Dox (1 μg/mL) for 48 hours and then harvested for immunoblotting (B), ChIP analyses of MYC binding to TFEB promoter (C), qRT-PCR analyses for MYC and TFEB (D), ChIP analyses of MYC and TFEB binding on the indicated TFEB target genes (E), and expression analyses of the same TFEB target genes by qRT-PCR (F). ChIP with isotype IgG antibody served as a negative control. Error bars in A and C–F indicate mean ± SEM of three independent assays. * in A and C–F, P < 0.05 when compared with the control group.