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. 2021 Apr 1;10:e67267. doi: 10.7554/eLife.67267

Figure 2. IGF-1 reduces mIAHP and sIAHP in IL-L5PNs.

(A) Representative current traces recorded from IL-L5PNs in response to an 800 ms depolarizing voltage pulse from −60 mV to 0 mV in the control condition (ACSF, top) and the presence of NVP-AEW541 (40 nM, bottom), before (black), and during 10 nM IGF-1 (red). (B) Bar diagram summarizing the normalized amplitude of mIAHP (n = 9 cells/5 animals; ACSF vs IGF-1 **p<0.01 and ns, non-significant, n = 7 cells/7 animals NVP vs NVP + IGF-1, Student's paired t-test). Mann–Whitney test, n = 9/7 cells ACSF vs NVP, ns. (C) Plot showing the time course of the peak amplitude of the mIAHP in ACSF (red) and in NVP-AEW541 before and during IGF-1 (n = 9 cells/7 animals; ACSF vs IGF-1 ***p<0.0001 and ns n = 7 cells/7 animals NVP vs NVP + IGF-1, Student's paired t-test). Mann–Whitney test, n = 9/7 cells IGF-1 vs NVP + IGF-1, ns. (D and E) Same as B and C respectively but for the sIAHP. (F) Same as A, but in the presence of GDPβs in the patch pipette. Note that only the sIAHP is modulated by IGF-1 under blockade of the G proteins. (G) Bar diagram summarizing the normalized amplitude of mIAHP (left) and normalized area of sIAHP (right) under GDPβs (n = 8 cells/4 animals; mIAHP ACSF vs IGF-1 ns, non-significant and sIAHP, n = 8 cells/4 animals ACSF vs IGF-1 *p<0.05, Student's paired t-test). See also Figure 2—figure supplement 1.

Figure 2—source data 1. Source data for Figure 2.
IGF-1 reduces mIAHP and sIAHP in IL-L5PNs.

Figure 2.

Figure 2—figure supplement 1. Time course of mIAHP and sIAHP.

Figure 2—figure supplement 1.

(A) Plot showing the time course of mIAHP (left) and sIAHP (right) in control conditions (ACSF) after applying 800 ms depolarizing pulses, respectively (n = 4 in each group). Note that the IAHPs are not modulated by the protocol used for their induction in the absence of IGF-1. (B) Plot showing the time course of mIAHP and the persistent effect of IGF-1 after washout; start at 0 min; n = 7 cells/6 animals, ACSF vs IGF-1 **p<0.01 Student'´s paired t-test; and start at 20 min after IGF-1 infusion n = 6 cells/4 animals, ACSF vs IGF-1 **p<0.01 Student's paired t-test. (C) Same as B but for the sIAHP. Note the LTD of IAHP was similar in both groups.
Figure 2—figure supplement 1—source data 1. Source data for Figure 2—figure supplement 1.
Time course of mIAHP and sIAHP.