Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | C57BL/6J | Janvier Labs | RRID:IMSR_JAX:000664 | |
| Cell line (Homo sapiens) | HCAEC, HUVEC | PromoCell | ||
| Transfected construct (Homo sapiens) | SERPINH1 (Homo sapiens), shRNA(#1) | TRC library database, Broad institute | TRCN0000003590 | Lentiviral construct to transfect and express the shRNA |
| Transfected construct (Homo sapiens) | SERPINH1 (Homo sapiens), shRNA(#2) | TRC library database, Broad institute | TRCN0000003594 | Lentiviral construct to transfect and express the shRNA |
| Transfected construct (Homo sapiens) | SERPINH1 (Homo sapiens), shRNA(#3) | TRC library database, Broad institute | TRCN0000003593 | Lentiviral construct to transfect and express the shRNA |
| Transfected construct (Homo sapiens) | SERPINH1 (Homo sapiens), shRNA(#4) | TRC library database, Broad institute | TRCN0000003591 | Lentiviral construct to transfect and express the shRNA |
| Transfected construct (Homo sapiens) | FUW-SERPIH1-Myc (Homo sapiens) | This paper | Lentiviral construct to transfect and express overexpress SERPINH1-Myc | |
| Biological sample (Homo sapiens) | Heart samples from donors | Helsinki University Hospital | ||
| Antibody | (Rat monoclonal), FITC-CD31 | Invitrogen | RM5201, RRID:AB_10373983 | FACS (1:100) |
| Antibody | (Rat monoclonal), Pacificblue-CD45 | Biolegend | 103125, RRID:AB_493536 | FACS (1:100) |
| Antibody | (Rat monoclonal), Pacificblue-Ter119 | Biolegend | 116231, RRID:AB_2149212 | FACS (1:100) |
| Antibody | (Rat monoclonal), PE-Cyanine7-CD140a | eBioscience | 25-1401, RRID:AB_2573399 | FACS (1:100) |
| Antibody | (Rat monoclonal), CD16/CD32 (Fc blocker) | BD Biosciences | 553142, RRID:AB_394657 | FACS (1:100) |
| Antibody | (Rat monoclonal), CD31 | BD Pharmingen | 553370, RRID:AB_394816 | Immunofluorescent (1:500) |
| Antibody | (Rabbit monoclonal), VEcadherin | Cell Signaling | 2500S, RRID:AB_10839118 | Immunofluorescent or western blotting (1:500) |
| Antibody | (Sheep Polyclonal), Tagln | R&D Biosystems | AF7886 | Immunofluorescent (1:500) |
| Antibody | (Mouse Monoclonal), c-MYC | Thermo Fisher | 13-2500, RRID:AB_2533008 | Immunofluorescent or western blotting (1:500) |
| Antibody | (Mouse Monoclonal), HSP47/SERPINH1 | Enzo Life Sciences | ADI-SPA-470-D, RRID:AB_2039239 | Immunofluorescent immunohistochemistry or western blotting (1:1000) |
| Antibody | (Rabbit Polyclonal), Collagen 1 | Abcam | ab34710, RRID:AB_731684 | Immunofluorescent (1:1000) |
| Antibody | (Mouse Monoclonal), aSMA | Sigma-Aldrich | A5228, RRID:AB_262054 | Immunofluorescent (1:500) |
| Antibody | (Mouse Monoclonal), GAPDH |
Millipore | CB1001, RRID:AB_2107426 | Western blotting (1:500) |
| Sequence-based reagent | hSERPINH1_F | This paper | SYBR green PCR primers | ATGAGAAATTCCACCACAAGATG |
| Sequence-based reagent | hSERPINH1_R | This paper | SYBR green PCR primers | GATCTTCAGCTGCTCTTTGGTTA |
| Sequence-based reagent | hCD31_F | This paper | SYBR green PCR primers | CTGCTGACCCTTCTGCTCTGTTC |
| Sequence-based reagent | hCD31_R | This paper | SYBR green PCR primers | GGCAGGCTCTTCATGTCAACACT |
| Sequence-based reagent | hCDH5_F | This paper | SYBR green PCR primers | CGTGAGCATCCAGGCAGTGGTAGC |
| Sequence-based reagent | hCDH5_R | This paper | SYBR green PCR primers | GAGCCGCCGCCGCAGGAAG |
| Sequence-based reagent | hTIE1_F | This paper | SYBR green PCR primers | ACCCGCTGTGAACAGGCCTGCAGAGA |
| Sequence-based reagent | hTIE1_R | This paper | SYBR green PCR primers | CTTGGCACTGGCTTCCTCT |
| Sequence-based reagent | hCYCLIND1_F | This paper | SYBR green PCR primers | GCGGAGGAGAACAAACAGAT |
| Sequence-based reagent | hCYCLIND1_R | This paper | SYBR green PCR primers | TGAGGCGGTAGTAGGACAGG |
| Sequence-based reagent | hTAGLN_F | This paper | SYBR green PCR primers | CGGTTAGGCCAAGGCTCTAC |
| Sequence-based reagent | hTAGLN_R | This paper | SYBR green PCR primers | CCAGCTCCTCGTCATACTTC |
| Sequence-based reagent | haSMA_F | This paper | SYBR green PCR primers | AAGCACAGAGCAAAAGAGGAAT |
| Sequence-based reagent | haSMA_R | This paper | SYBR green PCR primers | ATGTCGTCCCAGTTGGTGAT |
| Sequence-based reagent | hCD44_F | This paper | SYBR green PCR primers | TGGCACCCGCTATGTCGAG |
| Sequence-based reagent | hCD44_R | This paper | SYBR green PCR primers | GTAGCAGGGATTCTGTCTG |
| Sequence-based reagent | hVIM_F | This paper | SYBR green PCR primers | CGAGGAGAGCAGGATTTCTC |
| Sequence-based reagent | hVIM_R | This paper | SYBR green PCR primers | GGTATCAACCAGAGGGAGTGA |
| Sequence-based reagent | hNOTCH3_F | This paper | SYBR green PCR primers | ACCGATGTCAACGAGTGTCT |
| Sequence-based reagent | hNOTCH3_R | This paper | SYBR green PCR primers | GTTGACACAGGGGCTACTCT |
| Sequence-based reagent | hZEB2_F | This paper | SYBR green PCR primers | GAGGCGCAAACAAGCCAATC |
| Sequence-based reagent | hZEB2_R | This paper | SYBR green PCR primers | TCAGAACCTGTGTCCACTAC |
| Sequence-based reagent | hSLUG_F | This paper | SYBR green PCR primers | ACTCCGAAGCCAAATGACAA |
| Sequence-based reagent | hSLUG_R | This paper | SYBR green PCR primers | CTCTCTCTGTGGGTGTGTGT |
| Sequence-based reagent | hFN1_F | This paper | SYBR green PCR primers | CCATAGCTGAGAAGTGTTTTG |
| Sequence-based reagent | hFN1_R | This paper | SYBR green PCR primers | CAAGTACAATCTACCATCATCC |
| Sequence-based reagent | hVCAM1_F | This paper | SYBR green PCR primers | CGCAAACACTTTATGTCAATGTTG |
| Sequence-based reagent | hVCAM1_R | This paper | SYBR green PCR primers | GATTTTCGGAGCAGGAAAGC |
| Sequence-based reagent | hICAM1_F | This paper | SYBR green PCR primers | TGCCCTGATGGGCAGTCAAC |
| Sequence-based reagent | hICAM1_R | This paper | SYBR green PCR primers | CCCGTTTCAGCTCCTTCTCC |
| Sequence-based reagent | hHPRT1_F | This paper | SYBR green PCR primers | TGAGGATTTGGAAAGGGTGT |
| Sequence-based reagent | hHPRT1_R | This paper | SYBR green PCR primers | TCCCCTGTTGACTGGTCATT |
| Sequence-based reagent | hCDKN1A_F | This paper | SYBR green PCR primers | CAGCATGACAGATTTCTACC |
| Sequence-based reagent | hCDKN1A_R | This paper | SYBR green PCR primers | CAGGGTATGTACATGAGGAG |
| Sequence-based reagent | hCDKN2A_F | This paper | SYBR green PCR primers | AGCATGGAGCCTTCG |
| Sequence-based reagent | hCDKN2A_R | This paper | SYBR green PCR primers | ATCATGACCTGGATCGG |
| Sequence-based reagent | hIL6_F | This paper | SYBR green PCR primers | GCAGAAAAAGGCAAAGAATC |
| Sequence-based reagent | hIL6_R | This paper | SYBR green PCR primers | CTACATTTGCCGAAGAGC |
| Sequence-based reagent | hIL7_F | This paper | SYBR green PCR primers | TCGATCATTATTGGACAGC |
| Sequence-based reagent | hIL7_R | This paper | SYBR green PCR primers | AGGAAACACAAGTCATTCAG |
| Sequence-based reagent | hMMP10_F | This paper | SYBR green PCR primers | ACCAATTTATTCCTCGTTGC |
| Sequence-based reagent | hMMP10_R | This paper | SYBR green PCR primers | GTCCGTAGAGAGACTGAATG |
| Sequence-based reagent | hCXCL5_F | This paper | SYBR green PCR primers | ATTTGTCTTGATCCAGAAGC |
| Sequence-based reagent | hCXCL5_R | This paper | SYBR green PCR primers | TCAGTTTTCCTTGTTTCCAC |
| Sequence-based reagent | hANKRD1_F | This paper | SYBR green PCR primers | TGAGTATAAACGGACAGCTC |
| Sequence-based reagent | hANKRD1_R | This paper | SYBR green PCR primers | TATCACGGAATTCGATCTGG |
| Sequence-based reagent | hPLAT_F | This paper | SYBR green PCR primers | GGAATTCCATGATCCTGATAG |
| Sequence-based reagent | hPLAT_R | This paper | SYBR green PCR primers | TCCGGCAGTAATTATGTTTG |
| Sequence-based reagent | hPAI-1_F | This paper | SYBR green PCR primers | CGCAACGTGGTTTTCTC |
| Sequence-based reagent | hPAI-1_R | This paper | SYBR green PCR primers | CATGCCCTTGTCATCAATC |
| Sequence-based reagent | hNRARP | This paper | Taqman PCR probes |
Hs01104102_S1 |
| Sequence-based reagent | mCdh5 | This paper | Taqman PCR probes |
Mm00486938_m1 |
| Sequence-based reagent | mTie1 | This paper | Taqman PCR probes |
Mm00441786_m1 |
| Sequence-based reagent | mSerpinH1_F | This paper | SYBR green PCR primers | ATGTTCTTTAAGCCACACTG |
| Sequence-based reagent | mSerpinH1_R | This paper | SYBR green PCR primers | TCGTCATAGTAGTTGTACAGG |
| Sequence-based reagent | mVwa1_F | This paper | SYBR green PCR primers | GATGATCTTCCTATCATTGCC |
| Sequence-based reagent | mVwa1_R | This paper | SYBR green PCR primers | CAATTCCAGCACGTAGTAAC |
| Sequence-based reagent | mVim_F | This paper | SYBR green PCR primers | CTTGAACGGAAAGTGGAATCCT |
| Sequence-based reagent | mVim_R | This paper | SYBR green PCR primers | GTCAGGCTTGGAAACGTCC |
| Sequence-based reagent | mTgfbr2_F | This paper | SYBR green PCR primers | TCTTTTCGGAAGAATACACC |
| Sequence-based reagent | mTgfbr2_R | This paper | SYBR green PCR primers | GTAGCAGTAGAAGATGATGATG |
| Sequence-based reagent | mVash1_F | This paper | SYBR green PCR primers | CAAGGAAATGACCAAAGAGG |
| Sequence-based reagent | mVash1_R | This paper | SYBR green PCR primers | ACTGTTGGTGAGGTAAATTC |
| Sequence-based reagent | mSparc_F | This paper | SYBR green PCR primers | GAACCCACATGGCAAGTCTTA |
| Sequence-based reagent | mSparc_R | This paper | SYBR green PCR primers | AAAGCCCAATTGCAGTTGAGT |
| Sequence-based reagent | mTgfb1_F | This paper | SYBR green PCR primers | CTCCCGTGGCTTCTAGTGC |
| Sequence-based reagent | mTgfb1_R | This paper | SYBR green PCR primers | GCCTTAGTTTGGACAGGATCTG |
| Sequence-based reagent | mApln_F | This paper | SYBR green PCR primers | CAGGCCTATTCCCAGGCTCA |
| Sequence-based reagent | mApln_R | This paper | SYBR green PCR primers | CAAGATCAAGGGCGCAGTCA |
| Peptide, recombinant protein | Recominant human TGF- β | R&D Technologies | 240-B | 50 ng/ml |
| Commercial assay or kit | High-Capacity cDNA Reverse Transcription Kit | Applied biosystems | #4368814 | |
| Commercial assay or kit | FastStart Universal SYBR green master mix | Sigma-Aldrich | #04913914001 | |
| Commercial assay or kit | TaqMan gene expression master mix | Applied Biosystems | #4369016 | |
| Commercial assay or kit | SMARTer Stranded Total RNA-Seq Kit V2 – Pico Input Mammalian | Takara Bio, USA | ||
| Commercial assay or kit | SA-β-gal staining kit | Cell signaling technology | #9860 | |
| Commercial assay or kit | Click-iT EdU Alexa Fluor 594 staining kit | Thermo scientific | C10339 | |
| Chemical compound, drug | Hydrogen peroxide | Acros organics | AC202465000 | 200 μM |
| Software, algorithm | Chipster analysis platform (v3.12.2) | CSC, Finland | https://chipster.csc.fi | |
| Software, algorithm | Trimmomatic tool | Chipster, CSC, Finland | https://chipster.csc.fi/manual/trimmomatic.html | |
| Software, algorithm | HISAT2 package | Chipster, CSC, Finland | https://chipster.csc.fi/manual/hisat2.html | |
| Software, algorithm | HTSeq count tool | Chipster, CSC, Finland | https://chipster.csc.fi/manual/htseq-count.html | |
| Software, algorithm | DESeq2 Bioconductor package | Chipster, CSC, Finland | https://chipster.csc.fi/manual/deseq2-pca-heatmap.html | |
| Software, algorithm | PANTHER classification system (V.14.1) | http://www.pantherdb.org | ||
| Software, algorithm | VENNY 2.1 Venn-diagram analysis | BioinfoGP | https://bioinfogp.cnb.csic.es/tools/venny/ | |
| Software, algorithm | MetazSecKB knowledgebase | http://proteomics.ysu.edu/secretomes/animal/index.php | ||
| Software, algorithm | TargetP2.0 server | http://www.cbs.dtu.dk/services/TargetP/index.php | ||
| Software, algorithm | SecretomeP1.0 server | http://www.cbs.dtu.dk/services/SecretomeP-1.0/ | ||
| Software, algorithm | Image J software | NIH, Bethesda | https://imagej.nih.gov/ij/download.html | |
| Software, algorithm | SASP atlas | http://www.saspatlas.com | ||
| Software, algorithm | SeneQuest | https://senequest.net | ||
| Software, algorithm | Tabula Muris | https://tabula-muris.ds.czbiohub.org | ||
| Software, algorithm | EndoDB | https://endotheliomics.shinyapps.io/endodb/ | ||
| Software, algorithm | Endothelial cell atlas | https://endotheliomics.shinyapps.io/ec_atlas/ |