Skip to main content
. 2021 Apr 13;10:e61172. doi: 10.7554/eLife.61172

Figure 4. Rescue of segmental aneuploidy by single transgenes.

Figure 4.

(A) Df63C1-65A9/+ cells contribute ~50% of the adult eye. (B) Very few cells heterozygous for the overlapping Df63A3-65A9 were recovered. (C) Df63A3-65A9/+ cells contribute ~50% of the adult eye in the presence of the RpL28+ transgene. (D). Very few cells heterozygous for Df87B8-89B16 contribute to the adult eye. (E). Df87B8-89B16/+ cells contribute ~50% of the adult eye in the presence of the eIF2γ + genomic transgene. (F). Very few cells heterozygous for Df87B8-89E5 contribute to the adult eye. (G). Df87B8-89E5/+ cells contribute ~20% of the adult eye in the presence of the eIF2γ + genomic transgene. (H) Quantification of results for the RpL28 region shown in panels A-C. Data for Df63C1-65A9 is the same as that already shown in Figure 2A,B. (I) Quantification of results for the eIF2γ region shown in panels D-G. Statistics. Pairwise comparisons used the Mann-Whitney procedure with the Benjamini-Hochberg correction for multiple testing (see Supplementary file 2). N as indicated for each genotype. ns – difference not statistically significant (p>0.05). ** - difference highly significant (p<0.01). Genotypes: For 63C1-65A9, y w hsF; PBac{WH}f05041 P{XP}d02570 /FRT82B. For 63A3-65A9, y w hsF; PBac{WH}f01922 P{XP}d02570 /FRT82B and y w hsF; {RpL28+P3-DsRed}/+; PBac{WH}f01922 P{XP}d02570 /+; FRT82B/+. For 87B8-89B16, y w hsF/+; P{XP}d06796 PBac{WH}f04937 /FRT82B and y w hsF/+; P{ry+ Su(var3-9+) eIF2γ+}/+; P{XP}d06796 PBac{WH}f04937 /+. For 87B8-89E5, y w hsF/+; P{XP}d06796 PBac{WH}f00971 /FRT82B and y w hsF/+; P{ry+ Su(var3-9+) eIF2γ+}/+; P{XP}d06796 PBac{WH}f00971 /+.

Figure 4—source data 1. % eye white data.
* Indicates that 4x w+ cells indicative of FLP-mediated duplication were also observed in this specimen. Gaps between measurements separate replicate data.