Appendix 1—key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila melanogaster) | RpL28 | Flybase: FBgn0035422 | ||
| Gene (Drosophila melanogaster) | eIF2γ | Flybase: FBgn0263740 | ||
| Genetic reagent (D. melanogaster) | w11-18 | Hazelrigg et al., 1984; Lee et al., 2016 | FLYBASE: FBal0018186 | Bloomington Drosophila Stock Center #3605 |
| Genetic reagent (D. melanogaster) | Xrp1m2-73 | Lee et al., 2016 | FLYBASE:FBal0346068 | Bloomington Drosophila Stock Center #81270 |
| Genetic reagent (D. melanogaster) | rpS12G97D | Tyler et al., 2007 | FLYBASE:FBal0193403 | |
| Genetic reagent (D. melanogaster) | (Df3L)H99 | Abbott and Lengyel, 1991 | FLYBASE:FBab0022359 | Bloomington Drosophila Stock Center #1576 |
| Genetic reagent (D. melanogaster) | (M3R)w124 aka RpS32 | Ferrus, 1975; Abbott and Lengyel, 1991 | FLYBASE: FBal0011951 | |
| Genetic reagent (D. melanogaster) | hs-FLP | Struhl and Basler, 1993 | FLYBASE:FBtp0001101 | |
| Genetic reagent (D. melanogaster) | ey-FLP | Newsome et al., 2000 | FLYBASE:FBal0098303 | |
| Genetic reagent (D. melanogaster) | P{neoFRT}80B | Xu and Rubin, 1993 | FLYBASE:FBti0002073 | Bloomington Drosophila Stock Center #1988 |
| Genetic reagent (D. melanogaster) | P{neoFRT}82B | Xu and Rubin, 1993 | FLYBASE:FBti0002074 | Bloomington Drosophila Stock Center #2050, 2051 |
| Genetic reagent (D. melanogaster) | P{rpS12+8 kb} | Xu and Rubin, 1993; Kale et al., 2018 | FLYBASE:FBal0337985 | |
| Genetic reagent (D. melanogaster) | P{rpS12-G97D8kb} | Xu and Rubin, 1993; Kale et al., 2018 | FLYBASE:FBal0337986 | |
| Genetic reagent (D. melanogaster) | P{ry+ Su(var)3–9+ eIF2γ +} | Xu and Rubin, 1993; Tschiersch et al., 1994; Kale et al., 2018 | ||
| Genetic reagent (D. melanogaster) | P{DsRed; RpL28+} | This study | See Materials and methods; Dr. Nicholas Baker’s lab. | |
| Genetic reagent (D. melanogaster) | P535A-1-4 | Xie and Golic, 2004 | FLYBASE:FBal0138188 | Bloomington Drosophila Stock Center #6815 |
| Genetic reagent (D. melanogaster) | PBac{RB}CG11617e00462 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0162546 | Bloomington Drosophila Stock Center #17859 |
| Genetic reagent (D. melanogaster) | PBac{WH}MED15f04180 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBti0042319 | Bloomington Drosophila Stock Center #18739 |
| Genetic reagent (D. melanogaster) | P{XP}CG9016d08241 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0160858 | Bloomington Drosophila Stock Center #19290 |
| Genetic reagent (D. melanogaster) | P{XP}CG9003d09761 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0160860 | Bloomington Drosophila Stock Center #19321 |
| Genetic reagent (D. melanogaster) | P{XP}saltod09417 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0159854 | Bloomington Drosophila Stock Center #19315 |
| Genetic reagent (D. melanogaster) | P{XP}CG11200d02302 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0162606 | Bloomington Drosophila Stock Center #19173 |
| Genetic reagent (D. melanogaster) | P{XP}sobd06074 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0158622 | Bloomington Drosophila Stock Center #19230 |
| Genetic reagent (D. melanogaster) | PBac{WH}Urof04888 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0159557 | Bloomington Drosophila Stock Center #18814 |
| Genetic reagent (D. melanogaster) | PBac{RB}Ssb-c31ae02272 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0159728 | Bloomington Drosophila Stock Center #18032 |
| Genetic reagent (D. melanogaster) | PBac{RB}CG31898e03937 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0161732 | Bloomington Drosophila Stock Center #18211 |
| Genetic reagent (D. melanogaster) | PBac{WH}CG9582f00857 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0160790 | Bloomington Drosophila Stock Center #18378 |
| Genetic reagent (D. melanogaster) | PBac{WH}teif00157 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0159247 | Bloomington Drosophila Stock Center #18299 |
| Genetic reagent (D. melanogaster) | PBac{RB}CG13018e00535 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0162408 | Bloomington Drosophila Stock Center #17863 |
| Genetic reagent (D. melanogaster) | PBac{RB}Cpr51Ae03998 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0162723 | Bloomington Drosophila Stock Center #18221 |
| Genetic reagent (D. melanogaster) | PBac{WH}CG10384f04349 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0162694 | Bloomington Drosophila Stock Center #18762 |
| Genetic reagent (D. melanogaster) | PBac{WH}CG42260f00464 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0225307 | |
| Genetic reagent (D. melanogaster) | PBac{WH}Jafrac2f01922 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0160273 | Bloomington Drosophila Stock Center #18489 |
| Genetic reagent (D. melanogaster) | PBac{WH}CG17746f05041 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0162020 | Bloomington Drosophila Stock Center #18834 |
| Genetic reagent (D. melanogaster) | P{XP}Leashd06455 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0161383 | Bloomington Drosophila Stock Center #19240 |
| Genetic reagent (D. melanogaster) | P{XP}cud05983 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0158886 | Bloomington Drosophila Stock Center #19225 |
| Genetic reagent (D. melanogaster) | w1118; P{XP}d06796/TM6B, Tb1 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBti0042862 | Bloomington Drosophila Stock Center #19250 |
| Genetic reagent (D. melanogaster) | P{XP}CG10311d06928 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0162706 | Bloomington Drosophila Stock Center #19255 |
| Genetic reagent (D. melanogaster) | P{XP}d02570 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBti0054904 | |
| Genetic reagent (D. melanogaster) | P{XP}wrm1d02813 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0160902 | Bloomington Drosophila Stock Center #19182 |
| Genetic reagent (D. melanogaster) | P{XP}UGPd07256 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0159573 | Bloomington Drosophila Stock Center #19267 |
| Genetic reagent (D. melanogaster) | PBac{WH}CG14894f04937 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0162215 | Bloomington Drosophila Stock Center #18821 |
| Genetic reagent (D. melanogaster) | PBac{RB}Cad89De03186 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0160726 | Bloomington Drosophila Stock Center #18129 |
| Genetic reagent (D. melanogaster) | PBac{WH}Actn3f00971 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0162831 | Bloomington Drosophila Stock Center #18397 |
| Genetic reagent (D. melanogaster) | PBac{RB}qine03728 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0162275 | Bloomington Drosophila Stock Center #18186 |
| Genetic reagent (D. melanogaster) | PBac{RB}DPCoACe03144 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0175762 | Bloomington Drosophila Stock Center #18121 |
| Genetic reagent (D. melanogaster) | PBac{WH}KaiR1Df03502 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0161451 | Bloomington Drosophila Stock Center #18663 |
| Genetic reagent (D. melanogaster) | PBac{WH}TotCf01700 | Xu and Rubin, 1993; Thibault et al., 2004 | FLYBASE:FBal0159656 | Bloomington Drosophila Stock Center #18460 |
| Antibody | polyclonalRabbit anti-XRP1(short) | Francis et al., 2016 | (1:200) dilution | |
| Antibody | Polyclonal Rabbit anti-active-Dcp1 | Cell Signalling Technology | Cat #9578 | (1:50) dilution |
| Antibody | Polyclonal Donkey anti-Rabbit IgG, Cy3 conjugate | Jackson Immunoresearch | Cat # 711-165-152 | (1:200) dilution |
| Recombinant DNA reagent | P{DsRed; RpL28+} | This study | See Materials and methods; Dr. Nicholas Baker’s lab. | |
| Recombinant DNA reagent | pGE-attBTT-loxP-DsRed (pTL780) |
Blanco-Redondo and Langenhan, 2018 | Addgene Plasmid #115160 |