Fig. 3.

A 4-color panel test with (a) the 4 color panel for all cells between side scattering signal area (SSC-A) vs. forward scattering signal area (FSC-A), showing three groups of cells and gated for lymphocytes with count at 29% of the total cells; (b) forward scattering signal height (FSC–H) vs. forward scattering signal area (FSC-A) for lymphocytes and gated for singlet count at 96% of the total lymphocytes; (c) side scattering light vs. CD45-CF405L fluorescent light, showing CD45⁺ leukocyte count at 99% of the total singlet lymphocytes; (d) side scattering light vs. CD19-CF633 fluorescence light, showing CD19⁺ B-cell count at 12% of the singlet leukocytes; (e) side scattering light vs. CD3-FITC fluorescence light, showing CD3⁺ T-cell co-receptor count at 79% of the singlet leukocytes; and (f) CD4 PE-Cy7 fluorescence light vs. CD3-FITC fluorescence light, showing CD4⁺ T-helper cell count at 51% of the singlet leukocytes. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)