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. 2021 Apr 9;14:931–940. doi: 10.2147/JPR.S297442

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© 2021 Shang et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

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Estrogen downregulated rat chondrocytes NGF expression and release via ERα. (A) Rat primary chondrocytes were exposed to estrogen at a dose of 1 nM for 0, 12, 24, 48 or 72 hours. At 24 hours with 1 nM estrogen, expression of NGF mRNA was downregulated (0.7Ct, 0.55±0.13 fold) compared to the control. (B) Decrease in NGF protein levels were observed similarly to gene expression. (C) Rat primary chondrocytes were exposed to estrogen with or without ER inhibitors (MPP, PHTPP). Co-incubation with MPP, thereby preventing estrogen-ERα signaling could prevent estrogen-decreased NGF mRNA expression, while with PHTPP was not. (D) Co-incubation with MPP prevented estrogen-decreased NGF protein release. All data were shown as mean ± SEM from three independent experiments.

Notes: *P <0.05, **P <0.01.

Abbreviations: ELISA, enzyme-linked immunosorbent assay; NGF, nerve growth factor; MPP, a specific estrogen receptorα antagonist; PHTPP, a selective estrogen receptorβ antagonist.