Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| V500 Rat Anti-Mouse CD45 (30-F11) | BD Bioscience | Cat# 561487 |
| FITC Rat Anti-Mouse CD90.2 (53–2.1) | BD Bioscience | Cat# 553003 |
| Alexa Fluor® 700 Rat Anti-Mouse CD8a (53–6.7) | BD Bioscience | Cat# 557959 |
| BV786 Rat Anti-Mouse IFNγ (XMG1.2) | BD Bioscience | Cat# 563773 |
| PE-Cy™7 Rat Anti-Mouse TNFα (MP6-XT22) | BD Bioscience | Cat# 557644 |
| PE Mouse Anti-Human Granzyme B (GB11) | BD Bioscience | Cat# 561142 |
| BV786 Rat Anti-Mouse CD11b (M1/70) | BD Bioscience | Cat# 740861 |
| Armenian hamster Anti-Mouse CD3e (145–2C11) | Thermo Fisher | Cat# 14-0031-82; RRID: AB 467048 |
| Syrian hamster Anti-Mouse CD28 (37.51) | Thermo Fisher | Cat# 14-0281-82; RRID: AB 466413 |
| PE Mouse anti-mouse/rat XCR1 Antibody (ZET) | BioLegend | Cat# 148204; RRID: AB 2563842 |
| PE Mouse anti-OVA257–264 (SIINFEKL) peptide bound to H-2Kb (eBio25-D1.16) | Thermo Fisher | Cat# 12-5743-82; RRID: AB 925771 |
| Rabbit monoclonal Anti-Calreticulin (D3E6) | Cell Signaling Technology | Cat# 12238; RRID: AB 2688013 |
| Rabbit monoclonal Anti-ERp57 (G117) | Cell Signaling Technology | Cat# #2881; RRID: AB 2160840 |
| Rabbit monoclonal Anti-IRE1α (14C10) | Cell Signaling Technology | Cat# 3294 |
| Rabbit monoclonal Anti-GAPDH (D16H11) | Cell Signaling Technology | Cat# 5174; RRID: AB 11129865 |
| Rabbit monoclonal Anti -Na+,K+-ATPase (3010) | Cell Signaling Technology | Cat# 3010 |
| Rabbit monoclonal Anti-BiP (C50B12) | Cell Signaling Technology | Cat# 3177; RRID: AB 2119845 |
| Rabbit monoclonal Anti-LAMP1 (C54H11) | Cell Signaling Technology | Cat# 3243; RRID: AB 2134478 |
| Mouse Anti-GFP (4B10) | Cell Signaling Technology | Cat# 2955; RRID: AB_1196614 |
| Rabbit monoclonal Anti-AIF (D39D2) XP® mAb | Cell Signaling Technology | Cat# 5318 |
| Rabbit monoclonal Anti-CD9 (EPR2949) | Abcam | Cat# ab92726 |
| InVivoMab Anti-mouse CSF1R (CD115) antibody (AFS98) | Bio X Cell | Cat# BE0213; RRID: AB 2687699 |
| InVivoMAb rat IgG2a isotype control (2A3) | Bio X Cell | Cat# BE0089 |
| InVivoPlus Anti-mouse PD-L1 (10F.9G2) | Bio X Cell | Cat# BE0101, RRID: AB_10949073 |
| InVivoPlus rat IgG2b isotype control (LTF-2) | Bio X Cell | Cat# BP0090 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant Mouse STC1 | Creative Biomart Inc. | Cat# STC1–16117M |
| Mouse Recombinant M-CSF | Stem Cell Technologies | Cat# 78059 |
| Recombinant Mouse Flt-3 Ligand/FLT3L Protein | R&D Systems | Cat# 427-FL-025 |
| EnGen® Spy Cas9 NLS | NEB | Cat# M0646T |
| Ovalbumin Peptide (257–264) chicken | Sigma Aldrich | Cat# S7951 |
| Ovalbumin | Sigma Aldrich | Cat# A5503 |
| 3x DYKDDDDK Peptide | Thermo Fisher | Cat# A36806 |
| Critical Commercial Assays | ||
| Mouse IFN-gamma DuoSet ELISA | R&D Systems | Cat# DY485 |
| Mouse IFN-gamma ELISpot Kit | R&D Systems | Cat# EL485 |
| Human STC1 DuoSet ELISA | R&D Systems | Cat# DY2958 |
| Anti-PE MicroBeads | Miltenyi Biotec | Cat# 130-048-801 |
| pHrodo™ Red, SE | Thermo Fisher | Cat# P36600 |
| Pierce™ Cell Surface Protein Isolation Kit | Thermo Fisher | Cat# 89881 |
| Anti-FLAG Magnetic Agarose | Thermo Fisher | Cat# A36797 |
| Mito-Tracker™ Deep Red FM | Thermo Fisher | Cat# M22426 |
| Opti-prep™ Density Gradient Medium | Sigma Aldrich | Cat# D1556 |
| Mitochondria isolation kit (crude) | Thermo Fisher | Cat# 89874 |
| Deposited Data | ||
| Raw and analyzed data | This paper | GEO: GSE161813 |
| IP-based mass spectrometry | This paper | PXD023251 |
| Experimental Models: Cell Lines | ||
| Mouse cell line: B16-F10 | ATCC | CRL-6475 |
| Mouse cell line: LLC | ATCC | CRL-1642 |
| Mouse cell line: MC38 | (Tanikawa et al., 2012) | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: NOD.SCID γc deficient (NSG) | The Jackson Laboratory | JAX: 005557 |
| Mouse: C57BL/6J | The Jackson Laboratory | JAX: 000664 |
| Mouse: OT-I TCR transgenic mice | The Jackson Laboratory | JAX: 003831 |
| Mouse: Pdcd1−/− mice | (Nishimura et al., 1998) | N/A |
| Mouse: Batf3−/− (Batf3tm1Kmm/J) | The Jackson Laboratory | JAX: 013755 |
| Oligonucleotides | ||
| sgRNA targeting sequence: Mouse Stc1 #1: CUUGUCUCUUCCAGCUGAAG | Synthego | N/A |
| sgRNA targeting sequence: Mouse Stc1 #2: AGGCAGCGAACCACUUCAGC | Synthego | N/A |
| sgRNA targeting sequence: Mouse Stc1 #3: AGCCCCGCAGCCAACCUGCA | Synthego | N/A |
| sgRNA targeting sequence: Mouse Calr #1: UUUGGAUUCGACCCAGCGGU | Synthego | N/A |
| sgRNA targeting sequence: Mouse Calr #2: UUCGACCCAGCGGUUGGUCC | Synthego | N/A |
| sgRNA targeting sequence: Mouse Calr #3: CAGAUGCCUGGACCAACCGC | Synthego | N/A |
| Primer: mouse Stc1 forward: AGGAGGACTGCTACAGCAAGCT | IDT | N/A |
| Primer: mouse Stc1 reverse: TCCAGAAGGCTTCGGACAAGTC | IDT | N/A |
| Primer: mouse Inhba forward: TGCTGCTCAAGTGCCAATAC | IDT | N/A |
| Primer: mouse Inhba reverse: AGCAAAAGTCGTGTGGTTGC | IDT | N/A |
| Primer: mouse Cytl1 forward: TTCAGAGCCTGAGGATTCCTGT | IDT | N/A |
| Primer: mouse Cytl1 reverse: CTTCCGCACTCTGTCCTTCA | IDT | N/A |
| Primer: mouse Runx2 forward: TCGCCTCACAAACAACCACA | IDT | N/A |
| Primer: mouse Runx2 reverse: CTGCTTGCAGCCTTAAATATTCCT | IDT | N/A |
| Primer: mouse Metrnl forward: TAAGACTGTTGGTGCGGGAC | IDT | N/A |
| Primer: mouse Metrnl reverse: GCCTCGGACAACAAAGTCAC | IDT | N/A |
| Primer: mouse Dlg4 forward: GATGAAGACACGCCCCCTCT | IDT | N/A |
| Primer: mouse Dlg4 reverse: CTGCAACTCATATCCTGGGGCTT | IDT | N/A |
| Primer: mouse Plin2 forward: GCTCTCCTGTTAGGCGTCTC | IDT | N/A |
| Primer: mouse Plin2 reverse: AACAATCTCGGACGTTGGCT | IDT | N/A |
| Primer: mouse Gapdh forward: CATCACTGCCACCCAGAAGACTG | IDT | N/A |
| Primer: mouse Gapdh reverse: ATGCCAGTGAGCTTCCCGTTCAG | IDT | N/A |
| Recombinant DNA | ||
| Stc1 (NM_009285) Mouse Tagged ORF Clone | Origene™ Technologies | Cat# MR203105L1 |
| Software and Algorithms | ||
| BD FACSDiva™ Software | BD Bioscience | https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-software/flow-cytometry-acquisition/facsdiva-software |
| Graphpad Prism 6.0 software | GraphPad Software, Inc. | http://www.graphpad.com/scientific-software/prism/ |
| Hisat2 | (Kim et al., 2015) | |
| HTSeq | (Anders et al., 2015) | |
| DESeq2 | (Love et al., 2014) | |