A) Optic nerve axon counts, B) optic nerve axon densities (axon count normalized by nerve cross-sectional area), and C) nerve grades of rats 6 weeks after treatment with MB photocrosslinking (MB+Light). Control eyes were injected with HBSS and did not receive MB or light. Data points show mean ± standard deviation. For axon count and nerve density, n=5 for HBSS control and n=7 for MB+light due to limitations of axon counting software ability to analyze some sections. For nerve grade, n=8 for both HBSS control and MB+Light. * p<0.05, ** p<0.01, *** p<0.001.