Number of sections (span) in which each giant vacuole (GV) (n = 3302) appears was counted for each GV fully within the imaging field. A: Median span of GVs with I-pores (61 sections, IQR: 42 – 86) was significantly larger than GVs without I-pores (27, IQR: 18 – 47; P ≤ 0.01). B: In high-flow areas, median span of GVs with I-pores (60, IQR: 41 – 82) was significantly larger than GVs without I-pores (26, IQR: 17 – 45; P ≤ 0.01). C: In low-flow areas, median span of GVs with I-pores (66, IQR: 47 – 112) was significantly larger than GVs without I-pores (32, IQR: 21 – 53; P ≤ 0.01). D: In non-flow areas, median span of GVs with I-pores (62, IQR: 40 – 96) was significantly larger than GVs without I-pores (25, IQR: 17 – 44; P ≤ 0.01). E: Median span of GVs with multiple I-pores (80, IQR: 61 – 120) was significantly larger than GVs with a single I-pore (57, IQR: 39 – 82; P ≤ 0.01). F: The median span of Type I GVs was 22 sections (IQR: 15 – 32). Median span of Type II GVs was 31 sections (IQR: 19 – 54), and this was significantly larger than Type I (P ≤ 0.01). Median span of Type III GVs was 46 sections (IQR: 30 – 69), which was significantly larger than Type I and II (both P ≤ 0.01). Median span of Type IV GVs was 65 sections (IQR: 46 – 93), which was significantly larger than all other types (all P ≤ 0.01). Type I: no basal opening or I-pore; Type II: basal opening, no I-pore; Type III: with I-pore, no basal opening; Type IV: both basal opening and I-pore. **P ≤ 0.01