Table 2.

Counts of giant vacuoles observed and percentage of GVs with pores

Flow-Type Area	Eye	Count: GVs observed over entire surface area†	Count: GVs within imaging field‡	Count: GVs with I-pores‡	Percentage GVs with I-pores#
High-flow	1	1023	975	151	15.5%
	2	317	309	88	28.5%
	Total	1340	1284	239	18.6%
Low-flow	1	608	579	83	14.3%
	2	217	212	41	19.3%
	Total	825	791	124	15.7%
Non-flow	1	424	396	43	10.9%
	2	861	831	71	8.5%
	Total	1285	1227	114	9.3%
Overall		3450	3302	477	14.4%

^†These values include giant vacuoles (GVs) that were partially outside the imaging field on the left-hand side or the beginning of the block but exclude those on the right-hand side and end of block.

^‡These values only include GVs that were completely within the imaging field.

^#Percentages were calculated by dividing counts of GVs with I-pores by GVs within imaging field.