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. 2021 Apr 9;14:2449–2461. doi: 10.2147/OTT.S286813

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© 2021 Fu et al.

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(A) Apoptosis of HONE1 cells as revealed by flow cytometry after staining with Annexin V-FITC and propidium iodide. (B) Qualitative analysis of apoptosis employing Hoechst 33258 staining. The free drugs (alone or in combination) and cisplatin- and afatinib-loaded lipid–polymer hybrid nanoparticles were incubated with cancer cells for 24 h.