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. 2021 Apr 13;12:2194. doi: 10.1038/s41467-021-22503-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Read counts showing intestine-specific loading of miR-60 and promoter GFP fusion showing intestine-specific expression. Graph shows reads per million in indicated cell types of two biological replicate experiments, two bars. Scale bars, 50 µm. b Quantification (fluorescence intensity) of fat content using Oil Red O staining on wild-type and mir-60(n4947) starvation-recovered worms. Error bars represent +/− s.e.m of ten independent animals. P value represents a two-way unpaired Students t-test. a.u., arbitrary units. c Wild type or mir-60(n4947) animals after recovery from starvation. Scale bars, 1 mm. d Quantification of body area of cell-type-specific complemented mir-60(n4947). Percentages shown are the relative average body areas of animals compared to wild type. Error bars represent +/− s.e.m. P values represent one-way ANOVA with Tukey’s multiple comparisons test compared to wild type (black values) or mir-60(n4947) animals (green values). n ≥ 18 biologically independent animals for each strain tested.