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. 2021 Apr 13;11:8034. doi: 10.1038/s41598-021-86910-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of protein and RNA content of EVs isolated from different biofluids. (A) Quantity of total RNA extracted from CCM-EVs using UC and iSUF (n = 6; p < 0.05). (B) Comparison of proteins extracted from CCM-EVs using UC and iSUF (n = 6; p < 0.05). (C) Quantification of total RNA extracted from serum and urine EVs isolated by iSUF. (D) Quantification of EV proteins isolated from serum and urine EVs isolated by iSUF. (E) Western blot analysis of CD63 and CD9 expression in original CCM, CCM-EVs processed using iSUF and UC, original serum and urine, serum and urine-EVs processed using iSUF. The expression level of CD63 and CD9 was calculated using an equal mass (40 μg) of protein lysates from samples. (F). Total RNA of EVs isolated using different EV isolation methods. Black boxplots were the absolute amount of RNA isolated from 0.5 mL of serum. iSUF isolated the most RNA contents from serum EVs (n = 5; p < 0.05) (mean ± STD).