Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 14;6:148. doi: 10.1038/s41392-021-00535-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — GOLM1 restricts colitis and CAC through maintaining goblet cells and intestinal barrier. a Intestinal permeability was measured by concentration of FITC-dextran in mouse serum (the data are represented as the means ± SEM, n = 5; *P < 0.05, **P < 0.01, ***P < 0.0001; unpaired, two-tailed Student’s t test). b Representative MUC-2 staining of mouse colon sections obtained from mice treated with 2% DSS and sacrificed on indicated days. Scale bars, 100 μm. Quantification is shown in the histogram (the data are represented as the means ± SEM, n = 5; **P < 0.01, ****P < 0.0001; unpaired, two-tailed Student’s t test). c Representative PAS staining of mouse colon sections obtained from Golm1^−ΔIEC \and Golm1^−flox/flox mice. White arrows indicate innate mucus layer. Scale bars, 20 μm. Quantification is shown in the histogram (the data are represented as the means ± SEM, n = 5; *P < 0.05; unpaired, two-tailed Student’s t test). d Representative CD3 staining of mouse colon sections obtained from mice treated with 2% DSS and sacrificed on indicated days. Scale bars, 100 μm. e Relative mRNA expression levels of inflammatory mediators in the distal colon of DSS-treated mice determined by qRT-PCR. Relative expression reflects the fold change calculated by comparing with the average expression levels in untreated Golm1^−flox/flox mice (the data are represented as the means ± SEM, n = 5; P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; unpaired, two-tailed Student’s t test). f Representative PAS staining of mouse colon sections obtained from DSS-treated mice. Scale bars, 100 μm. Quantification is shown in the histogram (the data are represented as the means ± SEM, n = 5; **P < 0.01; unpaired, two-tailed Student’s t test). g Representative electron microscopy images of goblet cells and epithelial glycocalyx produced by goblet cells between the intestinal microvilli from the colons of Golm1^−ΔIEC and Golm1^−flox/flox mice. Scale bars, 100 nm (upper panel), 200 nm (lower panel). White arrows indicate mucopolysaccharide matrix (epithelial glycocalyx). h Relative mRNA expression levels of the indicated genes in IECs isolated from Golm1^−ΔIEC and Golm1^−flox/flox mice determined by qRT-PCR (the data are represented as the means ± SEM, n = 5; *P < 0.05, **P < 0.01; unpaired, two-tailed Student’s t test)