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. 2021 Apr 13;11:8002. doi: 10.1038/s41598-021-87061-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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1,25(OH)₂D3 and CYP11A1-derived secosteroids stimulate LXR activated ABCA1 gene expression in HaCaT keratinocytes. Relative expression of ABCA1 gene after treatment with 10^–7 M 1,25(OH)₂D3 and 20,23(OH)₂D3 for 6 (A) and 24 h (B). Values were retrieved from microarray data deposited at the NCBI GEO (GSE117351)⁴⁴. ChIP analysis performed on isolated nuclei from HaCaT keratinocytes treated with 1,25(OH)₂D3 using antibody against both LXRα and β, showed significant stimulation of the LXRα/β binding to the LXR-RE of the ABCA1 (C) but not the control gene, GADPH. Means ± SD from 3 independent experiments (D). QPCR quantification of ABCA1 in RNA from HaCaT keratinocytes (n = 3) treated with 20(OH)D3, 25(OH)D3, 1,25(OH)₂D3, 20(OH)L3, 20,22(OH)₂L3 and 24(OH)L3 (E). *p < 0.5 and **p < 0.01 by student t-test. The experiment was repeated 3 times. The heatmap was prepared using ClustVis software (https://biit.cs.ut.ee/clustvis/).

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