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. 2021 Apr 13;11:8085. doi: 10.1038/s41598-021-87526-y

Figure 3.

Figure 3

(a) Plasma (1 mL) from 9 healthy donors was subjected to centrifugation at 16, 000 × g for 10 min (squares) or not (circles) prior to the addition of Vn96 (100 µg) for EV isolation. DNA was extracted using the Qiagen Circulating Nucleic Acid Kit (Qiagen, cat # 55,114) and quantified with ddPCR using an RNASE P TaqMan Assay. (b) DNA, RNA and protein yields per mL of starting plasma. Yields of DNA and RNA (ng) on the left y-axis and protein (µg) on the right y-axis ± SEM per mL of starting plasma (n = 4).