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. 2021 Apr 13;12:2208. doi: 10.1038/s41467-021-22458-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Expansion of B1a cells (CD19⁺ IgM⁺ CD11b⁺ CD5⁺ ) in the peritoneal cavity, spleen and bone marrow of Dnmt3a^-/- mice (mean + SD). The top panel shows the gating strategy used to distinguish B1a and B1b cells. Five wild-type and Dnmt3a^-/- mice were analyzed in each group. Statistically significant differences are indicated with asterisks. b Number of bone marrow B2 cell precursors (Fractions A-C′, D, E, F) and mature splenic B2 cells (newly formed (NF), follicular (FO-I and FO-II), marginal zone precursor (MZP), and marginal zone (MZ) B cells) from 8 week old Dnmt3a^-/- mice and their littermate controls. The experiment was performed two times and a total of eight mice in each group were analyzed. The box and whiskers mark the 25th to 75th percentiles and the minimum or maximum values. Statistically significant differences (two-sided unpaired t-tests, FDR > 1%) are indicated with asterisks (p = 0.001104 (FO-I), p = 0.000087 (FO-II), p = 0.000175 (MZ)). c IgH repertoire of peritoneal B1a cells in wild-type and Dnmt3a^-/- mice at varying ages (top row). Each clonotype is represented by a distinctly colored rectangle, with an area proportional to the fraction of total reads. Kappa and lambda Ig light chain staining in B1a cells from wild-type and Dnmt3a^-/- mice at varying ages is also shown (bottom row). d Ki-67 levels in B1a cells from the peritoneal cavity and B2 cells from the spleens of 4-week-old wild-type and Dnmt3a^-/- mice. Source data are provided as a Source Data file.