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. 2021 Apr 13;11:8067. doi: 10.1038/s41598-021-86774-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Ex vivo rat sciatic nerve samples imaged with high-speed multimodal nonlinear and thermal microscopy. (A) Combined image of B-E. Images and corresponding high-speed line scans through the centers of the fields of view with (B) CARS at 2880 cm⁻¹. (C) SRS at 2880 cm⁻¹, (D) FluoroMyelin Green, (E) SHG. Red lines on graphs indicate the exposure of the tissue to 1875 nm light from an optical fiber. Light exposure causes a local temperature increase over 1 s (thermal images, (F) and (G)) yielding a corresponding decrease in nonlinear signals.