Table 3.
Validation of candidate SNPs by genomic DNA PCR amplification and sequencing.
| ID | Primer information | PCR size (bp) | Sequence polymorphism | |||
|---|---|---|---|---|---|---|
| Forward | Reverse | Pink | White | Purple | ||
| T1 | TGTGCTATCACACCATGTCTTCA | TTAGGGGTCAATCCTACGGTACT | 496 | T | C | C |
| T2 | GGTGCATCAGAAGAGAACATTCG | GCTAAGTCAGCTCCAACAAATCC | 449 | A | T | T |
| T3 | AGGTGGAGGTTTTACAATGGCA | CCCAACTCCAGCTTCTTTCCTA | 410 | T | W(T/A) | A |
| T4 | AGGGATTTATGCATCCAGCAGAT | TTCTTTCTTGTAATGCCCGCTTC | 465 | T | C | C |
| T5 | TATACATTTGCTGTGGCACCTCT | CCTCTCTCTCCACAACTCTGAAC | 398 | A | T | T |
| T6 | TCATGCATTTCAGTTTGCATGGT | AGTTTCTTGTGCTGTCCATCAAC | 350 | T | C | C |
| T7 | TCTCATCACCTTCAGCAGAATCC | GGAGGGAGTAATTAACGAGCCAA | 360 | C | Y(C/T) | T |
| T8 | GGGAAGAGTACTCGAATAGCTGG | GGTTCTCAAAATTAGGAGGGGGT | 535 | A | T | T |
| T9 | GGTCCGATGGCAAATGATACAAG | CCCACCACCCATAAGAACTACAA | 438 | C | T | C |